rabbit anti human ddit3  (Proteintech)

Journal: Cell Proliferation

Article Title: DDIT 3 overexpression increases odontoblastic potential of human dental pulp cells

doi: 10.1111/cpr.12104

Figure Lengend Snippet: Primer sequences for qRT‐PCR

Article Snippet: Samples were subsequently incubated in rabbit anti‐human DDIT3 antibody (1:50; Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4 °C overnight.

Techniques: Sequencing

Journal: Cell Proliferation

Article Title: DDIT 3 overexpression increases odontoblastic potential of human dental pulp cells

doi: 10.1111/cpr.12104

Figure Lengend Snippet: Immunofluorescent staining and endogenous DDIT3 expression in human dental pulp cells (HDPCs). (a) Immunofluorescent staining of DDIT3 in HDPCs without primary antibody (a‐1–3: DAPI staining, TRITC‐conjugated secondary antibody staining and merge). (b) Immunofluorescent staining of DDIT3 in wild‐type HDPCs (b‐1–3: DAPI staining, TRITC‐conjugated secondary antibody staining and merge). (c) Immunofluorescent staining showed increased nuclear accumulation of DDIT3 in TNFα‐challenged HDPCs (arrow, c‐1–3: DAPI staining, TRITC‐conjugated secondary antibody staining and merge). (d) mRNA expression of IL‐6, IL‐1β and DDIT3 in HDPCs after TNFα treatment. (e) Endogenous DDIT3 mRNA expression during odontoblastic differentiation detected by qRT‐PCR. (f) Endogenous DDIT3 protein expression during odontoblastic differentiation detected by Western blot. *Indicates a significant difference between two groups (P < 0.05). Scale bar = 50 μm.

Article Snippet: Samples were subsequently incubated in rabbit anti‐human DDIT3 antibody (1:50; Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4 °C overnight.

Techniques: Staining, Expressing, Quantitative RT-PCR, Western Blot

Journal: Cell Proliferation

Article Title: DDIT 3 overexpression increases odontoblastic potential of human dental pulp cells

doi: 10.1111/cpr.12104

Figure Lengend Snippet: Lentiviral infection of human dental pulp cells (HDPCs) and DDIT3 expression. (a and d) Cell image of HDPCs‐WT and GFP‐positive cells. (b and e) Cell image of HDPCs‐GFP and GFP‐positive cells. (c and f) Cell image of HDPCs‐DDIT3‐overexpression and GFP‐positive cells. (g) DDIT3 mRNA expression determined by qRT‐PCR in HDPCs‐WT, HDPCs‐GFP and HDPCs‐DDIT3‐overexpression groups. (h) DDIT3 protein expression determined by Western blot in HDPCs‐WT, HDPCs‐GFP and HDPCs‐DDIT3‐overexpression groups. *Indicates a significant difference between HDPCs‐GFP and HDPCs‐DDIT3‐overexpression groups (P < 0.05). Scale bar = 200 μm.

Article Snippet: Samples were subsequently incubated in rabbit anti‐human DDIT3 antibody (1:50; Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4 °C overnight.

Techniques: Infection, Expressing, Over Expression, Quantitative RT-PCR, Western Blot

Journal: Cell Proliferation

Article Title: DDIT 3 overexpression increases odontoblastic potential of human dental pulp cells

doi: 10.1111/cpr.12104

Figure Lengend Snippet: Proliferation ability and mineralization‐related genes expression in human dental pulp cells (HDPCs). (a) Cell proliferation. (b–g) ALP, Runx2, OSX, DSPP, DMP1 and OCN mRNA expression during odontoblastic differentiation at day 0, 7 and 14. (h) DSPP protein expression in HDPCs‐WT, HDPCs‐GFP and HDPCs‐DDIT3‐overexpression groups by Western blot at day 14. The histogram shows the quantification of gene expression and is presented as fold changes compared with HDPCs‐GFP group. *Indicates a significant difference between HDPCs‐GFP and HDPCs‐DDIT3‐overexpression groups (P < 0.05).

Article Snippet: Samples were subsequently incubated in rabbit anti‐human DDIT3 antibody (1:50; Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4 °C overnight.

Techniques: Expressing, Over Expression, Western Blot

Journal: Cell Proliferation

Article Title: DDIT 3 overexpression increases odontoblastic potential of human dental pulp cells

doi: 10.1111/cpr.12104

Figure Lengend Snippet: DDIT 3 overexpression increased the human dental pulp cells (HDPCs) odontoblastic differentiation. (a–c) ALP staining at day 7 in HDPCs‐WT, HDPCs‐GFP and HDPCs‐DDIT3‐overexpression groups. (e–g) Alizarin red staining of the cultured HDPCs at day 14 in HDPCs‐WT, HDPCs‐GFP and HDPCs‐DDIT3‐overexpression groups. (i–k) Von Kossa staining of the bone nodules formed at day 14 in HDPCs‐WT, HDPCs‐GFP and HDPCs‐DDIT3‐overexpression groups. (d) The percentage of ALP‐positive cells area. (h) Alizarin red absorbance at 590 nm. (l) Mineralized matrix areas. *Indicates a significant difference between HDPCs‐GFP and HDPCs‐DDIT3‐overexpression groups (P < 0.05). Scale bar = 500 μm.

Article Snippet: Samples were subsequently incubated in rabbit anti‐human DDIT3 antibody (1:50; Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4 °C overnight.

Techniques: Over Expression, Staining, Cell Culture